KMID : 0368420200630010011
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Journal of Plant Biology 2020 Volume.63 No. 1 p.11 ~ p.21
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MAP3K¥è1 is Involved in Abscisic Acid Signaling in Drought Tolerance and Seed Germination in Arabidopsis
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Jia Liguo
Chen Yuzhen Fan Mingshou Li Wenrao Zhang Jianhua
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Abstract
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Mitogen-activated protein kinase cascades play pivotal roles in mediating environmental stress responses and plant development. In this study, a loss-of-function mutant of Arabidopsis, map3k¥è1, exhibited wider stomatal openings, reduced root elongation, and increased seed germination rate compared with its wild type under exogenous abscisic acid (ABA) treatment. MAP3K¥è1 encodes a MAP kinase kinase kinase (MAP3K) with unknown function. Two overexpression lines of MAP3K¥è1 exhibited inhibited seed germination and narrowed stomata, which were aggravated by ABA treatment. Upregulation of MAP3K¥è1 also resulted in stronger drought tolerance, whereas map3k¥è1 was more sensitive to water deficiency, partially due to differences in the water-holding capacity of leaves. The MAP3K¥è1-overexpressing lines also showed a greater ability to maintain root elongation under exogenous ABA. Expression of MAP3K¥è1 was inhibited by ABA, H2O2, and methyl viologen treatments in roots. The MAP3K¥è1-overexpressing lines accumulated more ABA by promoting its biogenesis and inhibiting its catabolism, whereas the map3k¥è1 mutant accumulated less ABA, compared with wild-type plants. These findings indicate that MAP3K¥è1 promotes ABA accumulation to regulate stomatal movement, root elongation, and seed germination, while the ABA?H2O2 signaling module inhibits MAP3K¥è1 expression through feedback regulation.
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KEYWORD
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Abscisic acid, Arabidopsis, MAP3K¥è1, MAPK, Seed germination, Stomatal movement
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